CMB JOURNAL CLUB
LỊCH BÁO CÁO VÀ TÊN BÀI BÁO SẼ TRÌNH BÀY
COMPARATIVE PROTEOMICS OF TUMOR AND PAIRED NORMAL BREAST TISSUE HIGHLIGHTS POTENTIAL BIOMARKERS IN BREAST CANCER. (JC-20/6/2019)
Breast cancer is the most common type of cancer among women worldwide, and about 57,000 new cases are expected for the Brazilian population in 2015. Elucidation of protein expression and modification is essential for the biological understanding, early diagnosis and therapeutics of breast cancer. The main objectives of the study are comparison between the proteome of tumor and paired non-tumor breast cancer tissues, describing all identified proteins, highlighting the ones most differentially expressed and comparing the data with existing literature
NEXT‑GENERATION SEQUENCING IDENTIFIES NOVEL MITOCHONDRIAL VARIANTS IN PITUITARY ADENOMAS (JC-30/5/2019)
Disrupted mitochondrial functions and genetic variants of mitochondrial DNA (mtDNA) have been observed in different human neoplasms. Next-generation sequencing (NGS) can be used to detect even low heteroplasmy-level mtDNA variants. We aimed to investigate the mitochondrial genome in pituitary adenomas by NGS.
INVIVO GENOME EDITING OF THE ALBUMIN LOCUS AS PLATFORM FOR PROTEIN REPLACEMENT THERAPY
Site-specific genome editing provides a promising approach for achieving long-term, stable therapeutic gene expression. Genome editing has been successfully applied in a variety of preclinical models, generally focused on targeting the diseased locus itself; however, limited targeting efficiency or insufficient expression from the endogenous promoter may impede the translation of these approaches, particularly if the desired editing event does not confer a selective growth advantage.
DEVELOPMENT AND APPLICATIONS OF CRISPR-CAS9 FOR GENOME EDITING
Recent advances in genome engineering technologies based on the CRISPR-associated RNAguided endonuclease Cas9 are enabling the systematic interrogation of mammalian genome function. Analogous to the search function in modern word processors, Cas9 can be guided to specific locations within complex genomes by a short RNA search string.